Research Paper Volume 15, Issue 22 pp 13452—13470

Figure 5. PLB regulated ferroptosis by inhibiting chondrocytes catabolism via MAPK pathways. (A) The changes of mRNA expression were shown on volcano charts and bar chart. (B) The GO analysis. (C) The KEGG analysis. (D) Western blot analyses of ERK, p-ERK, JNK, p-JNK, P38 and p-P38 under PLB (2 μM) and H₂O₂ (500 μM) cultured conditions treatment and quantification. n = 3. (E) Western blot analyses of GPX-4, SLC7A11, ACSL4 and COX-2 under PLB, H₂O₂ and Mul-A cultured conditions treatment. (F) Quantitative western blot of Col2α1, ACAN, MMP-13 and Adamts-5 under PLB and H₂O₂ cultured conditions treatment. n = 3 (All quantified data are shown as mean ± SEM; #p < 0.05, ##p < 0.01, *p < 0.05, **p < 0.01, by one-way ANOVA followed by the Tukey-Kramer test, #: CON vs H₂O₂, *: H₂O₂ vs PLB+ H₂O₂).