Research Paper Volume 15, Issue 23 pp 13608—13627
Angelica gigas extract inhibits acetylation of eNOS via IRE1α sulfonation/RIDD-SIRT1-mediated posttranslational modification in vascular dysfunction
Figure 3. AGE inhibits oxidative stress in hyperlipidemic rats. (A) NADPH oxidase activity determined via lucigenin chemiluminescence assay. (B) 4-HNE + MDA level was measured as described in Materials and Methods. (C) Oxidized low-density lipoprotein (Ox-LDL) cholesterol content in plasma. (D) Representative images of DHE staining for ROS production and (E) quantification of DHE fluorescence intensity for ROS levels in aortas. (F) Quantification of mitoSOX fluorescence intensity for mitoROS levels in aortas. (G) Representative images of DHR staining for peroxynitrite in aortas and (H) quantification of DHR fluorescence intensity for peroxynitrite levels in aortas. (I) Nitrotyrosine analysis was performed as was described in Materials and Methods. Values are presented as mean ± SEM (n = 4~9, #p < 0.05 vs. the NCD group; *p < 0.05 vs. the HFD group). Abbreviations: NCD: normal chow diet; HFD: high-fat diet; AGE: Angelica gigas NAKAI extract.