Research Paper Volume 15, Issue 23 pp 13608—13627
Angelica gigas extract inhibits acetylation of eNOS via IRE1α sulfonation/RIDD-SIRT1-mediated posttranslational modification in vascular dysfunction
Figure 5. AGE improves endothelial cell functions in Ox-LDL-treated HUVECs. HUVECs were treated with decursin, or AGE, and then incubated with Ox-LDL for another 48 h. (A) NO production and (B) quantification of DAF-2DA fluorescence intensity. (C) NADPH oxidase activity was determined using lucigenin chemiluminescence assay. (D) Fluorescence intensity for mitoSOX levels was quantified in HUVECs. (E) Representative images of DHE staining for ROS production and (F) quantification of fluorescence intensity for ROS levels in HUVECs. #p < 0.05 compared to the control group; *p < 0.05 compared to the Ox-LDL only treated group. Values are presented as mean ± SEM (n = 3). Abbreviations: NO: nitric oxide; DAF-2DA: diaminofluorescein-2 diacetate; Scale bar: 100 μm.