Research Paper Volume 16, Issue 5 pp 4631—4653
miR-26a-5p inhibits the proliferation of psoriasis-like keratinocytes in vitro and in vivo by dual interference with the CDC6/CCNE1 axis
Figure 3. In vitro validation of the ability of miR-26a-5p to inhibit keratinocyte proliferation (A) miR-26a-5p expression in transfected HaCaT cells and HEKs compared with that in untreated control cells. (B) Proliferative ability (growth curve) of cells in each group. Dark blue line: untreated control; red line: cells induced with TNF-α; gray line: cells induced with TNF-α and transfected with miR-26a-5p. (C, D) Proportional changes in the cell cycle according to flow cytometry assays between TNF-α-induced cells transfected with or without miR-26a-5p. (E, F) mRNA and protein expression of miR-26a targets in HaCaT cells and HEKs. Group 1: Nontreated cells; Group 2: Cells with transfection reagent added; Group 3: Cells transfected with miR-26a. The data are presented as the mean ± SD (n = 5). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.