Research Paper Volume 17, Issue 3 pp 610—629

Mitochondrial oxidative stress or decreased autophagy in osteoblast lineage cells is not sufficient to mimic the deleterious effects of aging on bone mechanoresponsiveness

Figure 3. Sod2 deficiency in osteoblast-lineage cells does not alter load-induced increase in cortical bone mass. (A) mRNA levels measured by quantitative RT-PCR (n=5/genotype) and (B) mtROS levels (n=8/genotype) in bone marrow-derived stromal cell cultures from Osx1-Cre and Sod2^ΔOsx1 mice cultured for 7 days. Micro-CT measurements at 5 mm proximal to the tibiofibular junction (C) and at the midshaft (D) of the right (non-loaded) and left (loaded) tibiae from Osx1-Cre (n=7) and Sod2^ΔOsx1 (n=8) female mice. The tibiae of Osx1-Cre and Sod2^ΔOsx1 were loaded with -8.5N to achieve +1200με peak strain, following the same timeline as in Figure 1B. Bars represent mean ± SD. Data analyzed using two-tailed unpaired t-test or two-way mixed ANOVA; p-values adjusted by Holm-Sidak’s test. Int: Interaction; Gen: Genotype.