Research Paper Volume 17, Issue 3 pp 610—629
Article has an altmetric score of 47

Mitochondrial oxidative stress or decreased autophagy in osteoblast lineage cells is not sufficient to mimic the deleterious effects of aging on bone mechanoresponsiveness

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Figure 3. Sod2 deficiency in osteoblast-lineage cells does not alter load-induced increase in cortical bone mass. (A) mRNA levels measured by quantitative RT-PCR (n=5/genotype) and (B) mtROS levels (n=8/genotype) in bone marrow-derived stromal cell cultures from Osx1-Cre and Sod2ΔOsx1 mice cultured for 7 days. Micro-CT measurements at 5 mm proximal to the tibiofibular junction (C) and at the midshaft (D) of the right (non-loaded) and left (loaded) tibiae from Osx1-Cre (n=7) and Sod2ΔOsx1 (n=8) female mice. The tibiae of Osx1-Cre and Sod2ΔOsx1 were loaded with -8.5N to achieve +1200με peak strain, following the same timeline as in Figure 1B. Bars represent mean ± SD. Data analyzed using two-tailed unpaired t-test or two-way mixed ANOVA; p-values adjusted by Holm-Sidak’s test. Int: Interaction; Gen: Genotype.