Research Paper Advance Articles
Cisd1 synergizes with Cisd2 to modulate protein processing by maintaining mitochondrial and ER homeostasis
Figure 1. Generation of the Cisd1 knockout (KO) mouse model. (A) The process of generating the CIsd1 knockout mouse. (B) Southern blot analysis. Genomic DNA was digested by Nde I and probed with a 5’ flanking probe. (C) The genotyping results are able to identify wild-type, heterozygous and homozygous knockout mice. The primers for PCR genotyping are F1: 5’-GTGTGAGTGTGTCTGTACCTAG-3’, F2: 5’-CTGTCTACATCAGAGCAGAACAC-3’ and R: 5’-CAACTGCACCACAAATCATGTGAG-3’. (D) Protein expression of Cisd1 and CIsd2 in wild-type (WT), Cisd1 KO and Cisd2 KO mice. Abbreviations: B: brain; H: heart; K: kidney. Mouse age, 4–5 weeks old.