Research Paper Advance Articles

Figure 2. Shortened lifespan and severe growth retardation in the Cisd1&2 DKO mice. (A, B) The lifespan of the Cisd1 KO, Cisd2 KO and Cisd1&2 DKO male (A) and female (B) mice on B6/129 mixed background. Median survival of each genotype is present in the graph. Statistical analyses were calculated using Log-rank Mantal-Cox tests (Prism 8.0) and p < 0.001 (except WT vs. Cisd1 KO). (C) The growth curves of the male Cisd1 KO, Cisd2 KO and Cisd1&2 DKO mice. Body weight was monitored weekly from 2 weeks to 24 weeks old. The body weight of the Cisd2 KO and Cisd1&2 DKO mice were lower than WT mice from 4–5 weeks old, whereas the body weight of Cisd1 KO mice showed no obvious decrease compared to the WT mice. Data are shown as mean± SEM. (D) The photographs were of Cisd2 KO, Cisd1&2 DKO and WT male mice at 32 weeks of age. This photograph was taken from the dorsal view. (E) The growth curves of the female Cisd1 KO, Cisd2 KO and Cisd1&2 DKO mice. Body weight was monitored weekly from 2 weeks to 24 weeks old. The body weight of Cisd1 KO, Cisd2 KO and Cisd1&2 DKO mice were less than WT mice from 4 weeks old. Data was shown as mean± SEM. (F) The photographs were of Cisd2 KO, Cisd1&2 DKO and WT female mice at 26 weeks of age. These photographs were taken from the dorsal view.