Research Paper Advance Articles
Short-term moderate caloric restriction in the rhesus macaque attenuates markers of ovarian aging in select populations
Figure 7. Assessment of collagen I and III in histologic ovarian sections using Picrosirius red (PSR). Representative images of PSR stained ovarian tissue sections for (A) young control, (B) old control, (C) young calorie-restricted (CR), and (D) old calorie-restricted (CR). Corresponding representative images of the quantitative histology algorithm showing intensity of PSR staining, where low intensity pixels are shown in yellow, medium intensity pixels are shown in blue, and high intensity are shown in green for (E) young control, (F) old control, (G) young calorie-restricted (CR), and (H) old calorie-restricted (CR). Bar graphs showing quantification of (I) low intensity, (J) medium intensity, and (K) high intensity pixels. An N = 3 images per animal and N = 4–8 animals per experimental group were analyzed. Data are presented as mean ± SEM. Statistics were calculated using a two-way ANOVA with Sidak’s multiple comparison test. *p ≤ 0.05. **p 0.01. The scale bar is 1 mm.