Research Paper Advance Articles

Short-term moderate caloric restriction in the rhesus macaque attenuates markers of ovarian aging in select populations

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Figure 8. Hyaluronic acid binding protein localization in ovarian tissue sections. Representative fluorescent microscopy images of hyaluronic acid binding protein (HABP) showing localization of hyaluronic acid in ovarian tissue sections in (A) young control, (B) old control, (C) young calorie-restricted (CR), and (D) old calorie-restricted (CR) animals. Quantification of the positive pixels per area is shown in (E) with N = 3 images per animal and N = 4–8 animals per experimental group. Data are presented as mean ± SEM. Statistics were calculated using a two-way ANOVA with Sidak’s multiple comparison test. *p ≤ 0.05. The scale bar is 1 mm.