Research Paper Advance Articles

Figure 3. Spermidine increases lifespan and ameliorates age-related locomotive decline in both 2% and 12% diets independent of hypusination. (A) Survival analysis of isogenic w¹¹¹⁸ female flies, fed on 12% yeast and 2% yeast with and without addition of 5 mM spermidine. (B) Negative geotaxis of 30 days isogenic w¹¹¹⁸ female flies bred on either 12% or 2% with or without addition of 5 mM spermidine (n = 16 – 19 biological replicates. Each biological replicate contains around 15 female flies). (C) Survival analysis of isogenic w¹¹¹⁸ female flies and CG8005/+ animals, fed on 12% yeast and 2% yeast. (D) Negative geotaxis of 30-day old isogenic w¹¹¹⁸ flies and CG8005/+, bred on 12% yeast or 2% yeast (n = 15 – 16 biological replicates. Each biological replicate contains around 15 female flies). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. ns, not significant. Data are mean ± SEM. P-values were determined by the log rank test (A, C) and 2-way ANOVA with Tukey’s post multiple comparisons hoc test (B, D).