Research Paper Advance Articles
Senescence caused by telomerase inactivation in myeloid, mesenchymal, and endothelial cells has distinct effects on cancer progression
Figure 4. Tert KO in EC promotes pancreatic cancer metastasis linked with vasculature dysfunction and hypoxia. (A) Paraffin sections of representative KPC tumors from EC-Tert-KO and WT littermates subjected to IF showing GLUT1 expression in cancer cells (red arrows) and endomucin+ endothelium (GFP). (B) Paraffin sections of representative KPC tumors from indicated Tert-KO and WT littermates subjected to IF showing HIF1α expression in cancer cells (red arrows) and nuclear localization (purple arrows). Endomucin+ IF reveals blood vessels (green arrows) and isolated EC (green arrowheads). (C) Quantification of mean blood vessel length (A, B) and GLUT1 IF (A). Shown are mean +/- SEM, *P<0.001, **P<0.0005, ***P<0.0001, ANOVA with a post-hoc test. (D) Representative livers from EC-Tert-KO and WT littermates with pancreatic KPC tumors with liver metastases indicated (arrows). DNA is blue. Scale bar: 100 μm.