Research Paper Advance Articles

Figure 2. Impact of age-associated microbiome on gut homeostasis. (A) The gut morphology of aged female mice with FMT from young and old donors. (B) The intestinal length of female mice with reciprocal FMT. (C) The colon length of female mice with reciprocal FMT. (D) Immunofluorescent staining for claudin-5 in the intestines of female mice with reciprocal FMT (left panel). Relative claudin-5 expression in intestines of female mice with reciprocal FMT (right panel). (E) Immunohistochemical staining of IL-17A in the intestines of female mice with reciprocal FMT. (F) Quantification of IL-17A⁺ cells in the intestines of female mice with reciprocal FMT. Data in (B, C) were analyzed with Kruskal–Wallis followed by Dunn’s correction; data in (D, F) were analyzed with the two-sided Student’s t-test. Data are represented as mean ± SEM.