Correction Volume 17, Issue 7 pp 1909—1910

Figure 7. BRD4-induced inflammation reinforces the senescent phenotype via paracrine pathways. (A) THP-1 macrophages were cultured with LPS-induced senescent cell-derived conditioned medium for 24 h with or without siBRD4 or the BRD4 inhibitor JQ-1 (1 µM). Representative SA-β-gal was used to detect cell senescence, and Oil Red O staining was used to detect lipid accumulation in the cells. Scale bar, 50 µm. (B, C) The peritoneal macrophages from the Tert^−/− mice and human peripheral blood mononuclear cells (PBMCs) were cultured with the corresponding conditioned medium for 24 h. Representative SA-β-gal was used to detect cell senescence, and Oil Red O staining was used to detect lipid accumulation in the cells. Scale bar, 50 µm. The data all represent measured data presented as the mean ± SD. Comparisons between multiple groups were performed using one-way ANOVA, followed by Tukey’s post-hoc test. The experiment was repeated three times. Significant differences among different groups are indicated as ^**p < 0.01 vs. LPS CM; ^***p < 0.001 vs. Tert^−/− CM; ^****p < 0.0001 vs. LPS-PBMC CM.