Research Paper Volume 17, Issue 7 pp 1784—1809

Figure 4. The BM model secretes age-associated proteins. The BM model was statically cultured for five weeks. After two weeks, the BM cells were treated with either young or old human serum. On culture day 35, the BM cells were harvested, and the washed cell pellet analyzed using tandem LC-IMS-MS/MS proteomics. (A) Log2FC and −log2(p-value) of all significantly (p < 0.05) up (orange) or downregulated (turquoise) proteins in the BM with old serum compared to young serum. Proteins regulated in the same direction in at least 4 of 5 samples are depicted as well as either upregulated (red) or downregulated (blue). (B) Comparison of all regulated proteins to 2772 potentially secreted proteins according to the human protein atlas, creating an overlap of 233 proteins. (C) Go-Term analysis of down- (left) and up- (right) regulated overlapped proteins shown in (B). (D) Heatmap showing the log2FC of the overlapped 55 proteins in (E) depicting upregulated (red) and downregulated (blue) proteins with old serum. (E) Venn diagram showing the overlap of regulated proteins that belong to the human secretome (left) and secreted proteins that significantly change upon aging (right), resulting in 55 proteins shared between the two categories. (F) STRING protein network of the down- (left) and up- (right) regulated proteins from the 55 overlap proteins shown in (E). Expression by different BM cell types is highlighted with yellow circles (granulocytes), blue circles (progenitor cells) or violet circles (monocytes). Data were obtained from one experiment with 5 replicates.