Research Paper Advance Articles
Methylglyoxal-induced glycation stress promotes aortic stiffening: putative mechanistic roles of oxidative stress and cellular senescence
Figure 4. MGH-1 can increase aortic stiffness in young mouse aortas and gly-Low supplementation prevents it. (A) Study paradigm for the ex-vivo aortic stiffness measurement after 48-hour incubation with standard media (control), MGH-1, and MGH-1+Gly-Low. (B) Aortic elastic modulus for young intervention-naïve male mouse aortic rings after incubation (n=8/group). (C) Volcano plot showing the differential expressed genes (510 DEGs) with Padj < 0.05. (D) Gene ontology analysis of significantly upregulated (280) differentially expressed genes in response to Gly-Low treatment showing enriched biological processes. Panel (B) was analyzed using paired t-tests, as different media conditions were tested on aortas obtained from the same mouse. All values are in mean ± SEM, *p<0.05 vs. control; ♦p<0.05 vs. MGH-1 alone.