Research Paper Advance Articles
Methylglyoxal-induced glycation stress promotes aortic stiffening: putative mechanistic roles of oxidative stress and cellular senescence
Figure 7. Gly-Low lowers MGO-induced glycation stress and aortic stiffening in old mice. (A) Treatment paradigm for Gly-Low supplementation in 20-month-old male mice. (B) Aortic stiffness as measured by PWV between two study groups (n=8/group). (C) MGO levels in plasma of mice that received control or Gly-Low enriched diet (n=9–10/group). (D) Plasma MGH-1 levels (n=9/group). (E, F) Ex-vivo study paradigm focusing on aortic stiffness in intervention-naïve male old (24–26 months) mouse aortas after 48-hour incubation in standard media (control) and Gly-Low (n=8). Panels (B), (C), and (D) were analyzed using unpaired t-tests, while panel (F) was analyzed using a paired t-test, as different media conditions were tested on aortas obtained from the same mouse. All values are in mean ± SEM, *p<0.05 vs. old control.