Research Paper Advance Articles

Figure 1. Workflow of REVIVE. REVIVE has two different modes: (A) Based on a set of transcriptomic samples from perturbation experiments and corresponding control samples, REVIVE estimates the change in the predicted age and the enrichment of aging hallmarks in both groups. Perturbations that show a significant decrease in the predicted age and that rescue at least one aging hallmark are considered to be able to restore a more youthful transcriptional state. (B) In order to repurpose perturbations to restore youthful transcriptional states, REVIVE requires a set of differentially expressed genes between a young and old cell type or tissue sample and ranks perturbations based on their potential to revert the expression of the observed differentially expressed genes.