Research Paper Advance Articles
CD47 antisense oligonucleotide treatment improves glucose homeostasis and alleviates dyslipidemia in aged male mice
Figure 4. CD47 ASO treatment reduced de novo lipogenesis in 3T3-L1 adipocytes (after 15 days of differentiation). (A) Expression of the senescence marker, p21 in 15 days differentiated 3T3-L1 adipocytes (modeling adipocytes from old mice) compared to 5–7 days differentiated cells (modeling adipocytes from young mice); (B–D) CD47 or other gene expression in 7-day or 15-day differentiated adipocytes after 48 hours of treatment with the control ASO or CD47ASO (100 μM); (E) De novo lipogenesis measured in cell lysate and conditional medium from 15-day differentiated adipocytes. Data are represented as mean ± SEM (n = 3/group). **P < 0.01 compared to young group; *P < 0.05 and ***P < 0.001 compared to control ASO group.