Research Paper Volume 17, Issue 12 pp 2950—2988
Age-specific DNA methylation alterations in sperm at imprint control regions may contribute to the risk of autism spectrum disorder in offspring
Figure 2. Volcano plots of 450K data by age in sperm. Volcano plots of our age-association study on the 450K array. X-axis: Delta β-values (Δβ) representing age-associated changes in DNA methylation (after linear regression, adjusted for BMI and patient status). Y-axis: logarithmic transformation of the adjusted p-value (BH-method). Dashed line: indicates where the adjusted p-value is 0.05; dots above the dashed line represent CpG sites where sperm DNA methylation is significantly associated with male age. (A) All sites of the 450K are included (n = 482,287) and dots above the dashed line are age-associated DMCs (n = 14,622). (B–D) Volcano plots by subgroup of DNA methylation. Colored dots: significant age-related DMCs mapped to genes selected by their potential role in inheritance of ASD from father to child; these include: OTX1, PRDM16, PTPRN2, B4GALNT4, KCNQ1, KCNQ1OT1, DLGAP2, PLAGL1, GNAS, GRB10, MAGEL2, CDH24 and FBRSL1. Each colored dot represents one DMC; multiple dots with same color are allocated to the same gene.