Research Paper Volume 17, Issue 12 pp 2950—2988

Figure 6. Imprinted genes affected by age in sperm and our approach for biomarker selection to predict autism spectrum disorders in offspring. A summary of (predicted) imprinted genes linked to significant age-associated DMCs is ordered by expressed allele, and significance (results with smallest p-values are on top). Upper frame: 95 (predicted) imprinted genes linked to 271 DMCs within DNA methylation subgroups (Abbreviations: UM: unmethylated, mean β-value <0.2; HM: hemi-methylated, 0.20≤ mean β-value ≤0.80; FM: fully methylated, mean β-value >0.80). Lower frame: out of the 95 (predicted) imprinted genes (upper frame) 22 genes have been mapped to ICRs. In bold, genes involved in ASD. Right frame: summary of 27 ASD-related imprinted genes (due to probe cross-reactivity, the gene C6orf145 with a single DMC was not withheld). The following superscripts are used if at least one DMC had the following characteristic: *, at CpG Islands; ^PR, at promotor region; ↓ or ↑, an opposite direction in DNA methylation change was measured (increase or decrease in DNA methylation, at UM and FM methylated DMCs, respectively); ^s, at SNPs; ^cr, at a cross-reactive probe. M, maternally expressed genes; P, paternally expressed genes; I, isoform dependent.