Research Paper Volume 17, Issue 12 pp 2950—2988

Figure 8. DNA methylation by age at CpGs linked to DLGAP2 and SLC22A18AS. Estimates of DNA methylation in β-values by age for all significant CpG sites annotated to DLGAP2 (A–E) and SLC22A18AS (F–H) imprinted genes are shown; DNA methylation changes at these genes have been identified by at least three other age-related studies. Fitted regression lines are shown. Regression models included potential confounding factors (BMI and patient status); these were corrected for multiple testing (BH-method). The following CpG sites were annotated to DLGAP2: (A) cg03439898 (Delta β = 0.0085, p-value = 0.0025), (B) cg20076070 (Delta β = −0.011, p-value = 0.0056), (C) cg08082351 (Delta β = −0.0046, p-value = 0.7083), (D) cg12818159 (Delta β = 0.0059, p-value = 0.0272), and (E) cg24257495 (Delta β = 0.0049, p-value = 0.0038). The following CpG sites were annotated to SLC22A18AS: (F) cg08222610 (Delta β = −0.0153, p-value = 6.158515e-06), (G) cg26874323 (Delta β = −0.0097, p-value = 1.840627e-05), (H) cg23335134 (Delta β = −0.005, p-value = 6.076208e-05).