Research Paper Volume 18 pp 30—44

MT-RNR2 produces circular RNAs depleted in PBMCs of old cohort and in human diploid fibroblasts, WI-38. (A) Start and end of circMT-RNR2 from linear MT-RNR2. Position of primers to detect circular and linear MT-RNR2 are presented. (B) Circular RNA junctions identified from previous study (Jeck et al., 2013). (C) Abundance of circMT-RNR2 junction analyzed from total RNA-seq of PBMCs from young and old cohorts. (y-axis: count of circMT-RNR2 junction) (D) RT-qPCR analysis of circMT-RNR2 and western blot analysis of GRSF1 and HSP90 in WI-38 human diploid fibroblasts at different population doubling level (PDL).