Research Paper Volume 18 pp 30—44

Mitochondria-localized RNA-binding protein GRSF1 binds transcripts originated from mitochondrial genome. (A) GRSF1-GFP, expressed using the split GFP system (MTS-GFP1–10/GRSF1-GFP11), localizes to mitochondria, as confirmed by co-staining with anti-GRSF1 antibody and MitoTracker. DAPI was used to stain the nucleus. White arrows in the magnified view indicate regions of GRSF1 co-localization within mitochondria. The scale bar represents 20 μm. (B) Autoradiograph of ³²P-labeled RNA fragments identified from GRSF1 or IgG immunoprecipitation. (C) Example of GRSF1-bound mitochondrial transcripts with T-to-C conversion ratio and existence of circular RNA. (D) Common RNA-sequences from GRSF1 PAR-CLIP located in mitochondrial transcripts. The sequence motif and p-values are presented from. (E) Location of RNA fragments from GRSF1 PAR-CLIP located in MT-RNR2. T-to-C conversion ratio is denoted next to the coordinates.