Research Paper Volume 18 pp 30—44

circMT-RNR2 binds TCA cycle enzymes and accelerate production of succinate and fumarate in human diploid fibroblasts. (A) RT-qPCR of RNAs purified from immunoprecipitation of IgG or antibodies recognizing SUCLG1 and SDHA. (B) Concentration of Fumarate and alpha-ketoglutarate after deletion of MT-RNR2 from mitochondrial genome. (C) RT-qPCR analysis of circMT-RNR2 and western blot analysis of GRSF1 and HSP90 in WI-38 cells after transfection with the indicated combinations of empty vector, circMT-RNR2, control shRNA, and GRSF1 shRNA. (D) Colorimetric levels of succinate and fumarate after transfection of circMT-RNR2 and GRSF1 shRNA plasmid.