Research Paper Volume 18 pp 30—44

circMT-RNR2 suppresses senescence and preserve mitochondrial homeostasis. (A) RT-qPCR levels of p16 and p21 mRNAs as a marker of cellular senescence in WI-38 cells after transfection with the indicated combinations of empty vector, circMT-RNR2, control shRNA, and GRSF1 shRNA. (B) RT-qPCR levels of mitochondrial transcripts in WI-38 cells after transfection with the indicated combinations of empty vector, circMT-RNR2, control shRNA, and GRSF1 shRNA. Abbreviation: N.D.: Not Detected. (C) Possible model of circMT-RNR2 function scaffolding SUCLG1/G2 and SDHA/B/C/D to accelerate TCA cycle in mitochondria.