Research Paper Volume 18 pp 67—81
P38 MAPK is involved in epigenetic regulation of fibrotic genes in replication induced senescence in lung fibroblasts
α-SMA and Col3A1 protein expression in response to TGF-β1 with or without p38 MAPK inhibitor SB202190. IMR90 cells were subjected to p38 MAPK inhibitor alone or, treated with TGF-β1 (2ng/ml) at 2h (A, B) or 24h (C, D) for densitometry with or without pretreatment for 2h of SB202190 (10μM). β-actin was used as a loading control. T indicates TGF-β1; SB20, SB202190. Results are average of at least three independent experiments. Bar graphs indicate mean ± standard errors (SE). * p<0.05, compared to the vehicle control (Ctrl) within the same group, #p<0.05 compared to TGF-β1 only group.