Research Paper Volume 18 pp 82—99
D, L-Buthionine-(S, R)-sulfoximine recapitulates the anti-obesity effects of sulfur amino acid restriction without the associated deleterious effects on bone in male mice
Unlike the SAAR diet, BSO does not decrease trabecular bone mineral density. (A) Representative images of the micro-CT scanning of trabecular bone in tibiae show that the SAAR diet lowered multiple measures of trabecular bone density, including (B) the ratio of bone volume to total volume (SAAR < CD), (C) connectivity density (SAAR < CD), and (D) trabecular number (SAAR < CD). However, the SAAR diet increased the (E) trabecular space (SAAR > CD) but did not affect (F) trabecular thickness (SAAR = CD). As we hypothesized, NAC reversed all the SAAR-induced changes to the levels observed in the CD group, while BSO did not affect any of these parameters (SAAR < [CD = NAC] in figures B, C and D; SAAR > [NAC = CD] in figure E). Note: One-way ANOVA followed by Tukey’s multiple comparison tests was used to find group-wise differences (*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001; bars and error bars represent means and standard errors of the mean; n = 7/group).