Research Paper Volume 18 pp 82—99
D, L-Buthionine-(S, R)-sulfoximine recapitulates the anti-obesity effects of sulfur amino acid restriction without the associated deleterious effects on bone in male mice
The SAAR diet and BSO exert opposing effects on osteoblast number. Photomicrographs of the proximal tibia (40X magnification) showing osteoblasts on bone surfaces (red arrowheads), osteoid (yellow arrowheads), and bone marrow adipocytes (green circles in the adipocytes) are shown in (A). Consistent with the changes in bone mineral density, the SAAR diet decreased the number of (B) osteoblasts (SAAR < CD) and (C) osteoclasts (SAAR < CD). NAC reversed the effects of the SAAR diet on osteoblasts ((SAAR < [CD = NAC]) but not on osteoclasts (SAAR = NAC = CD). The SAAR diet also decreased (D) osteoid thickness and (E) osteoid volume; NAC reversed both. BSO did not affect any of these measures. Note: Sample sizes and statistical methods are similar to those in