Research Paper Volume 18 pp 369—379
Cross species activity of TERT human telomerase component
hTERT expression in mouse fibroblasts induces cell senescence. Mouse embryonic fibroblasts were transduced either with pLOC-empty or pLOC-hTERT, and samples were taken at day 1 and at day 6 post-infection. (A–D) hTERT (A) and the senescence markers p19ARF (Cdkn2A gene) (B), p16Ink4A (Cdkn2A gene) (C) and p21 (Cdkn1A gene) (D) transcriptional expression was analyzed by RT-q-PCR.