Figure 1.Methylene blue (MB) treatment does not alter stemness or cell cycle progression in human hair follicle stem cells (HFSCs). (A) Representative immunofluorescence images showing CD34 and K15 expression in control (mock-treated) and MB-treated HFSCs (Scale Bar:10μm). (B) Representative Western blot analysis of CD34 and K15 protein levels in control and MB-treated HFSCs. (C) Quantification of CD34 and K15 band intensities normalized to β-actin, based on two independent biological replicates. ns: not significant. (D) Flow cytometric analysis of cell cycle distribution showing comparable proportions of HFSCs in G0/G1, S, and G2/M phases between control and MB-treated groups (N=3). ns: not significant.
