Figure 4.The individual and combined effects of MB, Vitamin A (Vit A), and Vitamin C (Vit C) on β-catenin expression in HFSCs. (A) Representative immunoblots of total and active β-catenin in HFSCs treated for 15 days in Control, MB (100nM), Vit A (100nM), MB (100nM) +Vit A (100nM), Vit C (100μM), and MB (100nM) +Vit C(100μM) treated HFSCs for 10 days.(B) Densitometric quantification of active/total β-catenin ratios from (A) shows a significant increase following MB treatment alone. Neither Vit A nor Vit C, alone or in combination with MB, further enhances β-catenin activation beyond MB treatment. Notably, β-catenin activation is partially suppressed in the MB + Vit A and MB + Vit C groups. (***p<0.0001).
