Figure 5.MB promotes β-catenin activation and exhibits synergistic effects with minoxidil in HFSCs. (A) HFSCs were treated with different concentrations of minoxidil (1 μM, 2.5 μM, and 5 μM) for 5 days, and cell viability was assessed using the XTT assay. The 1 μM concentration of minoxidil showed the most significant benefit for HFSC growth and was selected for subsequent experiments. (B) Representative immunoblots showing total and active β-catenin levels in HFSCs treated for 15 days with 1 μM minoxidil, with or without MB co-treatment. (C) Densitometric quantification of active/total β-catenin ratios from (B) reveals significant activation by MB alone and further synergistic enhancement in the MB + minoxidil combination group. (*p<0.0101, *** p<0.0001, and **** p<0.0001).
