Figure 6.Co-culture transfer experiments of senescent-derived EVs onto myoblasts. (A) Schematic diagram of myoblasts cultured with EVs derived from senescent (SEV) or non-senescent (NSEV) myoblasts. Relative mRNA expression of senescence genes (B) Trp53 and (C) Cdkn1a within myoblasts 48h following the transfer of NSEV or SEV. Relative mRNA expression of top DE miRNA gene targets (D) Pten and (E) Mdm2. Relative mRNA expression of SASP genes (F) Il33 and (G) Cyr61. Data are means ± SD. * denotes a significant difference from the NSEV-T group (p<0.05). SEV-T: Senescent EV-treated myoblasts; NSEV-T: Non-senescent EV-treated myoblasts.
