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Research Paper Volume 11, Issue 14 pp 4990—5007
Long noncoding RNA FALEC inhibits proliferation and metastasis of tongue squamous cell carcinoma by epigenetically silencing ECM1 through EZH2
- 1 Department of Oral Surgery, Stomatological Hospital, Southern Medical University, Guangzhou 510280, People’s Republic of China
- 2 Department of Stomatology, Shunde Hospital, Southern Medical University, Foshan 528300, People’s Republic of China
- 3 Department of Radiation Oncology, Affiliated Cancer Hospital and Institute of Guangzhou Medical University, Guangzhou 510280, People’s Republic of China
Received: April 17, 2019 Accepted: July 10, 2019 Published: July 23, 2019
https://doi.org/10.18632/aging.102094How to Cite
Copyright © 2019 Jia et al. This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY) 3.0 License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Tongue squamous cell carcinoma (TSCC), the most common epithelial cancer identified in the oral cavity, has become one of the most common malignancies across the developing countries. Increasing evidence indicates that long non-coding RNAs (lncRNAs) serve as important regulators in cancer biology. The focally amplified long non-coding RNA in epithelial cancer (FALEC) was found downregulated in the tissues of tongue squamous cell carcinoma (TSCC) and was predicted to present a good prognosis by bioinformatics analysis. Experiments indicated that FALEC knockdown significantly increased the proliferation and migration of TSCC cells both in vitro and in vivo; however, FALEC overexpression repressed these malignant behaviors. RNA pull-down and RNA immunoprecipitation demonstrated that FALEC could recruit enhancer of zeste homolog 2 (EZH2) at the promoter regions of extracellular matrix protein 1 (ECM1), epigenetically repressing ECM1 expression. The data revealed that FALEC acted as a tumor suppressor in TSCC and may aid in developing a novel potential therapeutic strategy against TSCC.
Abbreviations
lncRNAs: long noncoding RNAs; FALEC: focally amplified long non-coding RNA in epithelial cancer; TSCC: tongue squamous cell carcinoma; RT-qPCR: Reverse transcription-quantitative polymerase chain reaction; RIP: RNA immunoprecipitation; EZH2: enhancer of zeste homolog 2; ChIP: Chromatin immunoprecipitation; H3K27me3: histone H3 lysine 27 trimethylation; ECM1: Extracellular matrix protein 1; TCGA: the cancer Genome Atlas; GEO: Gene Expression Omnibus; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; ATCC: American Type Culture Collection; IHC: immunohistochemistry; PRC2: Polycomb repressive complex 2; EMT: epithelial–mesenchymal transition; ITGB4: integrin β4.