Research Paper Volume 11, Issue 22 pp 10385—10408

Rotating magnetic field delays human umbilical vein endothelial cell aging and prolongs the lifespan of Caenorhabditis elegans

Jiangyao Xu 1, *, , Kan Liu 1, *, , Tingting Chen 1, 2, , Tianying Zhan 1, , Zijun Ouyang 1, , Yushu Wang 1, , Wen Liu 3, , Xiaoyun Zhang 1, , Yang Sun 3, , Gaixia Xu 2, , Xiaomei Wang 1, ,

  • 1 Base for International Science and Technology Cooperation: Carson Cancer Stem Cell Vaccines R&D Center, Shenzhen Key Lab of Synthetic Biology, Department of Physiology, School of Basic Medical Sciences Shenzhen University, Shenzhen 518055, China
  • 2 Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, School of Biomedical Engineering, Shenzhen University, Shenzhen 518055, China
  • 3 State Key Laboratory of Pharmaceutical Biotechnology, Department of Biotechnology and Pharmaceutical Sciences, School of Life Sciences, Nanjing University, Nanjing 210023, China
* Equal contribution

received: July 23, 2019 ; accepted: November 8, 2019 ; published: November 22, 2019 ;

https://doi.org/10.18632/aging.102466
How to Cite

Copyright © 2019 Xu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

The biological effects of magnetic fields are a research hotspot in the field of biomedical engineering. In this study, we further investigated the effects of a rotating magnetic field (RMF; 0.2 T, 4 Hz) on the growth of human umbilical vein endothelial cells (HUVECs) and Caenorhabditis elegans. The results showed that RMF exposure prolonged the lifespan of C. elegans and slowed the aging of HUVECs. RMF treatment of HUVECs showed that activation of adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPK) was associated with decreased mitochondrial membrane potential (MMP) due to increased intracellular Ca2+ concentrations induced by endoplasmic reticulum stress in anti-aging mechanisms. RMF also promoted the health status of C. elegans by improving activity, reducing age-related pigment accumulation, delaying Aβ-induced paralysis and increasing resistance to heat and oxidative stress. The prolonged lifespan of C. elegans was associated with decreased levels of daf-16 which related to the insulin/insulin-like growth factor signaling pathway (IIS) activity and reactive oxygen species (ROS), whereas the heat shock transcription factor-1 (hsf-1) pathway was not involved. Moreover, the level of autophagy was increased after RMF treatment. These findings expand our understanding of the potential mechanisms by which RMF treatment prolongs lifespan.

Abbreviations

RMF: Rotating magnetic field; C. elegans: Caenorhabditis elegans; AMPK: adenosine 5′-monophosphate (AMP)-activated protein kinase; HUVEC: human umbilical vein endothelial cell; MMP: mitochondrial membrane potential; IIS: insulin/insulin-like growth factor signaling; ROS: reactive oxygen species; hsf-1: heat shock transcription factor-1; Aβ: β-amyloid; DR: dietary restriction; ETC: electron transport chain.