Research Paper Volume 12, Issue 3 pp 2530—2544
Circular RNA expression in isoproterenol hydrochloride-induced cardiac hypertrophy
- 1 Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou City, Henan 450001, P. R. China
- 2 Medical Research Center, Peking University Third Hospital, Beijing 100191, P. R. China
- 3 Department of Pharmacology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou City, Henan 450001, P. R. China
- 4 School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou City, Henan 450001, P. R. China
- 5 Biobank of Peking University Third Hospital, Beijing 100191, P. R. China
received: May 3, 2019 ; accepted: January 12, 2020 ; published: February 5, 2020 ;https://doi.org/10.18632/aging.102761
How to Cite
Copyright © 2020 Yang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Circular RNA (circRNA) is a novel class of noncoding RNAs, and the roles of circRNAs in the development of cardiac hypertrophy remain to be explored. Here, we investigate the potential roles of circRNAs in cardiac hypertrophy. By circRNA sequencing in left ventricular specimens collected from 8-week-old mice with isoproterenol hydrochloride-induced cardiac hypertrophy, we found 401 out of 3323 total circRNAs were dysregulated in the hypertrophic hearts compared with the controls. Of these, 303 circRNAs were upregulated and 98 were downregulated. Moreover, the GO and KEGG analyses revealed that the majority of parental gene of differentially expressed circRNAs were not only related to biological process such as metabolic process and response to stimulus, but also related to pathway such as circulatory system and cardiovascular diseases. On the other hand, total 1974 miRNAs were predicted to binding to these differentially expressed circRNAs, and the possible target mRNAs of those miRNAs were also predicted and analyzed in terms of functional annotation. Finally, we identified that ANF and miR-23a are downstream targets of circRNA wwp1, suggesting that circRNA wwp1 exerts inhibitory roles of cardiac hypertrophy via down-regulation of ANF and miR-23a, which underlying the potential mechanisms whereby circRNA regulates cardiac hypertrophy.