Abstract

Background: MiRNA can be involved in regulating tumor genesis and development through regulating the major genes expression and regulating corresponding pathways. Here, we investigated the function and mechanisms of miR-483-3p in glioma progression.

Results: We found that miR-483-3p was increased in glioma cells and tissues. Silencing of miR-483-3p attenuated glioma progression and stemness. STAT3 activation which was induced by IL6 mediated the transcription of miR-483-3p gene.

Conclusion: The present study indicated miR-483-3p acting as a tumorigenesis gene regulated by IL6/STAT3 axis, thus providing more experimental basis for clinical treatment of glioma.

Methods: qRT-PCR was performed to measure miR-483-3p level. Transwell assay, wound healing assay, flow cytometry and immunofluorescence are used to detect the role of miR-483-3p in migration, invasion, epithelial-mesenchymal transition (EMT) and stemness in glioma cells. Western blot analysis was performed to analyze protein level. Luciferase assay was performed to examine the interaction between miR-483-3p and STAT3.