Research Paper Advance Articles
Transcriptional regulation of miR-483-3p mediated by IL-6/STAT3 axis promoted epithelial-mesenchymal transition and tumor stemness in glioma
- 1 Department of Neurosursery, China-Japan Union Hospital of Jilin University, Changchun, Jilin Province, China
- 2 Department of Lymphovascular Surgery, China-Japan Union Hospital of Jilin University, Changchun, Jilin Province, China
Received: April 16, 2020 Accepted: July 6, 2020 Published: November 4, 2020
https://doi.org/10.18632/aging.103761How to Cite
Copyright: © 2020 Xu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Background: MiRNA can be involved in regulating tumor genesis and development through regulating the major genes expression and regulating corresponding pathways. Here, we investigated the function and mechanisms of miR-483-3p in glioma progression.
Results: We found that miR-483-3p was increased in glioma cells and tissues. Silencing of miR-483-3p attenuated glioma progression and stemness. STAT3 activation which was induced by IL6 mediated the transcription of miR-483-3p gene.
Conclusion: The present study indicated miR-483-3p acting as a tumorigenesis gene regulated by IL6/STAT3 axis, thus providing more experimental basis for clinical treatment of glioma.
Methods: qRT-PCR was performed to measure miR-483-3p level. Transwell assay, wound healing assay, flow cytometry and immunofluorescence are used to detect the role of miR-483-3p in migration, invasion, epithelial-mesenchymal transition (EMT) and stemness in glioma cells. Western blot analysis was performed to analyze protein level. Luciferase assay was performed to examine the interaction between miR-483-3p and STAT3.