Research Paper Volume 12, Issue 21 pp 22078—22094

Prognostic risk signature based on the expression of three m6A RNA methylation regulatory genes in kidney renal papillary cell carcinoma

Zhuolun Sun1, , Changying Jing2, , Chutian Xiao1, , Tengcheng Li1, , Yu Wang1, ,

  • 1 Department of Urology, Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510630, China
  • 2 Eye Institute of Shandong University of Traditional Chinese Medicine, Jinan 250002, China

Received: April 1, 2020       Accepted: August 25, 2020       Published: November 7, 2020
How to Cite

Copyright: © 2020 Sun et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


In this study, we investigated the prognostic significance of the expression of N6-methyladenosine (m6A) RNA methylation regulatory genes in kidney renal papillary cell carcinoma (KIRP). RNA-sequencing data analysis showed that 14 of 20 major m6A RNA methylation regulatory genes were differentially expressed in the KIRP tissues from The Cancer Genome Atlas (TCGA) database. We constructed a prognostic risk signature with three m6A RNA methylation regulatory genes, IGF2BP3, KIAA1429 and HNRNPC, based on the results from univariate and LASSO Cox regression analyses. Multivariate Cox regression analysis confirmed that the risk score based on the three-gene prognostic risk signature was an independent predictive factor in KIRP. The overall survival of high-risk KIRP patients was significantly shorter than the low-risk KIRP patients. Expression of the three prognostic risk-related genes correlated with the AJCC and TNM stages of KIRP patients from TCGA and GEPIA datasets. ROC curve analysis showed that the three-gene prognostic risk signature precisely predicted the 1-year, 3-year and 5-year survival of KIRP patients. These findings demonstrate that expression of three prognostic risk-related m6A RNA methylation regulatory genes accurately predicts survival outcomes in KIRP patients.


AJCC: The American Joint Committee on Cancer; DFS: Disease free survival; HR: Hazard ratio; KIRP: Kidney renal papillary cell carcinoma; LASSO: least absolute shrinkage and selection operator; m6A: N6-methyladenosine; OS: overall survival; PCA: Principal component analysis; PPI: protein–protein interaction; ROC: Receiver operating characteristic; TCGA: The Cancer Genome Atlas.