Research Paper Advance Articles

Protective effect of DLX6-AS1 silencing against cerebral ischemia/reperfusion induced impairments

Xiamin Hu1, , Zifei Xiang2, , Wei Zhang3, , Zhijun Yu2, , Xiaoming Xin1, , Rong Zhang2, , Youping Deng4, , Qiong Yuan2, ,

  • 1 College of Pharmacy, Shanghai University of Medicine and Health Sciences, Shanghai, China
  • 2 Institute of Pharmaceutical Innovation, Hubei Province Key Laboratory of Occupational Hazard Identification and Control, College of Medicine, Wuhan University of Science and Technology, Wuhan, Hubei Province, China
  • 3 China Resources and WISCO General Hospital, Wuhan, Hubei Province, China
  • 4 Bioinformatics Core, Department of Complementary and Integrative Medicine, University of Hawaii John A. Burns School of Medicine, Honolulu, HI 96813, USA

Received: April 2, 2020       Accepted: August 14, 2020       Published: November 18, 2020      

https://doi.org/10.18632/aging.104070
How to Cite

Copyright: © 2020 Hu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

In the present study, we investigated the role of lncRNA mus distal-less homeobox 6 antisense 1 (DLX6-AS1) during cerebral impairment induced by stroke. DLX6-AS1 levels were upregulated during ischemia/reperfusion (I/R) and downregulation of DLX6-AS1 reduced acute injury and ameliorated long-term neurological impairments induced by cerebral I/R in mice. Additionally, silencing of DLX6-AS1 significantly decreased the neuronal apoptosis in vivo and in vitro. Furthermore, inhibition of miRNA-149-3p led to enhance the apoptosis, which confirmed that DLX6-AS1 could sponge miR-149-3p. Finally, BOK was predicted to be the target of miR-149-3p using TargetScanVert software. And the silencing of DLX6-AS1 inhibited BOK expression both in vivo and in vitro, which was reversed by a miR-149-3p inhibitor. At meantime, BOK promoted OGD/R induced apoptosis in N2a cells. Therefore, this suggests that miR-149-3p sponging by DLX6-AS1 may lead to cerebral neuron I/R-induced impairments through upregulation of apoptotic BOK activity, which offers a new approach to the treatment of stroke impairment.

Abbreviations

BOK: Bcl-2-related ovarian killer; CCA: common carotid artery; ceRNAs: competing endogenous RNAs; DLX6-AS1: DLX6 antisense RNA 1; ECL: enhanced chemiluminescence; GTEx: genotype-tissue expression; HRP: Corresponding horseradish peroxidase; Hsa: Homo sapiens; lncRNAs: long non-coding RNAs; I/R: ischemia/reperfusion; LV: lentivirus; MCA: middle cerebral artery; MCAO: middle cerebral artery occlusion; miRNAs: microRNAs; Mmu: Mus musculus; mNSS: modified neurological severity score; ncRNAs: non-coding RNAs; N2a: Neuro2a; OGD: oxygen glucose deprivation; OGD/R: oxygen-glucose deprivation/reoxygenation; PMSF: phenylmethylsulfonyl fluoride; siRNAs: small-interfering RNA; snoRNAs: small nucleolar RNAs; tRNAs: transfer RNAs; TUNEL: terminal deoxynucleotidyl transferase dUTP nick-end labeling.