Research Paper Volume 13, Issue 2 pp 2941—2958
Up-regulation of miR-204 inhibits proliferation, invasion and apoptosis of gallbladder cancer cells by targeting Notch2
- 1 Department of Pathology, Zibo Central Hospital, Zibo 255000, Shandong Province, China
- 2 Department of Pathology, Zibo Fourth People’s Hospital, Zibo 255067, Shandong Province, China
- 3 The Third Ward of Oncology Department, Zibo Central Hospital, Zibo 255000, Shandong Province, China
- 4 The First Ward of Gastroenterology Department, Zibo Central Hospital, Zibo 255000, Shandong Province, China
Received: July 24, 2020 Accepted: December 3, 2020 Published: January 13, 2021https://doi.org/10.18632/aging.202444
How to Cite
Copyright: © 2021 Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Gallbladder carcinoma (GC) is an extremely malignant gastrointestinal tumor, but relevant mechanisms are still under investigation. MicroRNA (miR) is differentially expressed in a variety of tumors. Here we explored miR-204 in patients with GC and related mechanisms. A GSE104165 chip was downloaded from the gene expression omnibus (GEO) for analysis. The qRT-PCR assay was used for quantifying miR-204 and Notch2 in the serum and tissues of the patients, and the patients were followed up for 3 years to analyze independent factors of prognosis. The CCK8, transwell, and flow cytometry assays were applied for analyzing proliferation, invasion, as well as apoptosis of cells, and the dual luciferase reporter (DLR) assay was adopted for determining the association of miR-204 with Notch2. MiR-204 was low in patients with GC, and it might serve as a diagnostic indicator for GC. In addition, patients with low e MiR-204 usually faced high rates of III+IV stage, distant metastasis, and low differentiation, and also showed a poor prognosis. DLR assay verified the targeted binding of miR-204 to Notch2 mRNA.