Research Paper Volume 13, Issue 14 pp 18404—18422

Expression mode and prognostic value of FXYD family members in colon cancer

Ming Jin1, *, , Hui Zhang2, *, , Jun Yang3, , Zhen Zheng2, , Kaitai Liu2, ,

  • 1 The Affiliated Lihuili Hospital, Ningbo University, Ningbo, China
  • 2 Department of Radiation Oncology, The Affiliated Lihuili Hospital, Ningbo University, Ningbo, China
  • 3 Ningbo Diagnostic Pathology Center, Ningbo, China
* Equal contribution

Received: February 26, 2021       Accepted: June 29, 2021       Published: July 15, 2021
How to Cite

Copyright: © 2021 Jin et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


The FXYD gene family comprises seven members that encode a class of small-membrane proteins characterized by an FXYD motif and interact with Na+/K+-ATPase. Until now, the expression patterns and prognostic roles of the FXYD family in colon cancer (CC) have not been systematically reported. Gene expression, methylation, clinicopathological features and the prognoses of CC patients were obtained from The Cancer Genome Atlas (TCGA) database. The expression feature and prognostic values of FXYD members were identified. Gene set enrichment analysis (GSEA) was performed to explore the potential mechanism underlying the function of the FXYD family in CC. Tumor Immune Estimation Resource (TIMER) and CIBERSORT analysis were used to assess the correlations between FXYD family members and tumor immune infiltrating cells (TIICs). FXYD family members were differentially expressed in CC except for FXYD2. FXYD2, FXYD3 and FXYD4 were revealed as independent prognostic factors for recurrence, while FXYD3 and FXYD7 were identified as prognostic factors for survival according to univariate and multivariate analyses with Cox regression. GSEA revealed that FXYD family members were involved in complicated biological functions underlying cancer progression. TIMER and CIBERSORT analyses showed significant associations between FXYD family genes and TIICs. The present study comprehensively revealed the expression mode and prognostic value of FXYD members in CC, providing insights for further study of the FXYD family as potential clinical biomarkers in CC.


CC: colon cancer; TCGA: The Cancer Genome Atlas; PPI: protein-protein interaction; GSEA: Gene set enrichment analysis; TIICs: tumor immune infiltrating cells; TIMER: Tumor Immune Estimation Resource; PFS: progression-free survival; OS: overall survival; GDC: Genomic Data Commons; HTSeq: high-throughput sequencing; FPKM: fragments per kilobase of transcript per million mapped reads; RT: radiotherapy.