Research Paper Volume 13, Issue 14 pp 18527—18544
LncRNA CCAT1 promotes prostate cancer cells proliferation, migration, and invasion through regulation of miR-490-3p/FRAT1 axis
- 1 Department of Urology, Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu, China
- 2 Department of Urology, Suzhou Kowloon Hospital Shanghai Jiao Tong University School of Medicine, Suzhou 215021, Jiangsu, China
- 3 Department of Urology, Changhai Hospital, Naval Medical University, Shanghai 200433, Yangpu, China
- 4 Department of Urology, Taixing People's Hospital, Taixing 225400, Jiangsu, China
Received: September 29, 2020 Accepted: April 29, 2021 Published: July 28, 2021https://doi.org/10.18632/aging.203300
How to Cite
Copyright: © 2021 Cai et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Prostate cancer (PCa) is a prevalent cancer in males, with high incidence and mortality. Recent studies have shown the crucial role of long non-coding RNA (lncRNA) in PCa. Here, we aimed to explore the functional roles and inner mechanisms of lncRNA CCAT1 in PCa cells. qRT-PCR results showed that CCAT1 was upregulated in PCa tissues and cells. Functional assays demonstrated that CCAT1 knockdown suppressed cell proliferation, migration, invasion, yet promoted apoptosis, while CCAT1 promotion showed the opposite results. We also found that CCAT1 negatively regulated miR-490-3p expression and subsequently regulated FRAT1 expression. Inhibition of miR-490-3p or up-regulation of FRAT1 reversed the suppressive effects of CCAT1 knockdown on the PCa cells. In conclusion, CCAT1 regulated FRAT1 expression through miR-490-3p and then promote the PCa cells proliferation, migration, and invasion, which reveals the oncogenic function of CCAT1 in PCa progress.
CCAT1: colon cancer associated transcript 1; CRC: colorectal cancer; EMT: epithelial-mesenchymal transition; FBS: fetal bovine serum; lncRNA: long non-coding RNA; MALAT1: metastasis-associated lung adenocarcinoma transcript1; miRNA: microRNA; PCa: prostate cancer; PCA3: prostate cancer antigen 3; PCGEM1: prostate cancer gene expression marker 1; qRT-PCR: Quantitative real-time PCR; siRNA: small interfering RNAs.