Research Paper Volume 14, Issue 15 pp 6202—6226

SIAH1-mediated RPS3 ubiquitination contributes to chemosensitivity in epithelial ovarian cancer

Lu Chen1,2, *, , Wujiang Gao1,2, *, , Chunli Sha1,2, , Meiling Yang5, , Li Lin1,2, , Taoqiong Li1,2, , Hong Wei2, , Qi Chen2, , Jie Xing1,2, , Mengxue Zhang1,2, , Shijie Zhao2, , Wenlin Xu2, , Yuefeng Li3, , Xiaolan Zhu1,2,4, ,

  • 1 Reproductive Medicine Center, The Fourth Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu, China
  • 2 Department of Central laboratory, The Fourth Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu, China
  • 3 Department of Radiology, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu, China
  • 4 International Genome Center of Jiangsu University, Zhenjiang, Jiangsu, China
  • 5 Obstetrics and Gynecology, The First People’s Hospital of Nantong City, Nantong, Jiangsu, China
* Equal contribution

Received: April 28, 2022       Accepted: July 12, 2022       Published: August 8, 2022
How to Cite

Copyright: © 2022 Chen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


The E3 ligase SIAH1 is deregulated in human cancers and correlated with poor prognosis, but its contributions to chemoresistance in epithelial ovarian cancer (EOC) are not evident. Herein we found that SIAH1 was decreased in EOC tumour tissues and cell lines and negatively correlated with the RPS3 levels. SIAH1 overexpression suppressed tumour cell growth, colony formation, invasion, metastasis, and cisplatin resistance in vivo and in vitro. SIAH1 promoted RPS3 ubiquitination and degradation using the RING-finger domain, and these steps were required for RPS3 localization to the cytoplasm, which led to subsequent NF-κB inactivation and thereby conferred chemosensitivity. Moreover, ectopic expression of RPS3 or depletion of RPS3 ubiquitination mediated by SIAH1 via the K214R mutant significantly impaired cisplatin-induced tumour suppression in cells stably expressing SIAH1. Together, our findings reveal a tumour suppressor function of SIAH1 and provide evidence showing that the SIAH1-RPS3-NF-κB axis may act as an appealing strategy for tackling treatment resistance in EOC.


EOC: Epithelial ovarian cancer; SIAH1: seven in absentia homologue 1; RPS3: Ribosomal protein S3; NF-κB: nuclear factor kappa B; cDDP: cisplatin; NcoR: nuclear receptor co-repressor; TRAF: TNF receptor associated factor; c-Myb: c-MYB proto-oncogene; MDR: Multidrug resistance; IC50: Half maximal inhibitory concentration; Co-IP: Co-immunoprecipitation; IP: Immunoprecipitation; WB: Western blotting; LC-MS/MS: Liquid Chromatography-Mass Spectrometry/Mass Spectrometry.