Research Paper Volume 14, Issue 18 pp 7408—7415
CircKIF4A promotes non-small cell lung cancer proliferation and metastasis through MiR-1238/CLDN14 axis
- 1 Department of Thoracic Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China
- 2 Department of Ophthalmology Surgery, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, China
- 3 Department of Cardiothoracic Surgery, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, China
Received: June 9, 2022 Accepted: August 25, 2022 Published: September 12, 2022
https://doi.org/10.18632/aging.204276How to Cite
Copyright: © 2022 Luo et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
As the leading cause of cancer-related death worldwide, non-small-cell lung cancer (NSCLC) is still in need of improved therapeutic strategies. CircKIF4A has been found to be involved in the progression of multiple cancers while its role in NSCLC remains unclear. To investigate the functions of circKIF4A, we assessed the expression of circKIF4A in NSCLC cells and tissues and performed experiments to determine the detailed functions of circKIF4A in NSCLC, including migration and proliferation. We found CircKIF4A expressed more heavily in the cells and tissues of NSCLC patients, and functional studies showed that inhibition of circKIF4A reduced NSCLC cells metastasis and proliferation. Furthermore, we seek to identify the underlying regulatory effect of circKIF4A in NSCLC. Studies revealed that circKIF4A sponged miR-1238 to promote NSCLC progression by up-regulating claudin14 (CLDN14) expression. In conclusion, circKIF4A is a potential diagnostic and therapeutic target in the circKIF4A/miR-1238/CLDN14 axis that plays an important role in NSCLC progression.
Abbreviations
CLDN14: claudin14; circRNAs: Circular RNAs; NSCLC: Non-small-cell lung cancer; IHC: Immunohistochemistry; CCK-8: Cell counting kit-8; qRT-PCR: quantitative real-time PCR; RIP: RNA immunoprecipitation; ncRNAs: non-coding RNAs.