Research Paper Volume 15, Issue 8 pp 2852—2862

A chronic wound model to investigate skin cellular senescence

Saranya P. Wyles1, , Parisa Dashti1, , Tamar Pirtskhalava2, , Burak Tekin3, , Christina Inman2, , Lilian Sales Gomez4, , Anthony B. Lagnado4, , Larissa Prata2, , Diana Jurk2, , João F. Passos2, , Tamar Tchkonia2, , James L. Kirkland2, ,

  • 1 Department of Dermatology, Mayo Clinic, Rochester, MN 55905, USA
  • 2 Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN 55905, USA
  • 3 Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA
  • 4 Department of Medicine, Division of General Internal Medicine, Mayo Clinic, Rochester, MN 55905, USA

Received: December 28, 2022       Accepted: April 3, 2023       Published: April 21, 2023
How to Cite

Copyright: © 2023 Wyles et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Wound healing is an essential physiological process for restoring normal skin structure and function post-injury. The role of cellular senescence, an essentially irreversible cell cycle state in response to damaging stimuli, has emerged as a critical mechanism in wound remodeling. Transiently-induced senescence during tissue remodeling has been shown to be beneficial in the acute wound healing phase. In contrast, persistent senescence, as observed in chronic wounds, contributes to delayed closure. Herein we describe a chronic wound murine model and its cellular senescence profile, including the senescence-associated secretory phenotype.


SASP: senescence-associated secretory phenotype; ECM: extracellular matrix; IACUC: Institutional Animal Care and Use Committee; ATZ: 3-amino-1,2,4-trizole; MSA: mercaptosuccinic acid; ISH: in situ hybridization; SMS: senescence messaging secretome.