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Research Paper|Volume 16, Issue 1|pp 169—190

Impact of macrophage differentiation on hematopoietic function enhancement by Shenzhu ErKang Syrup

Yuan Li1,2, Meng Teng2, Hongxin Yang2, Siyu Li2, Xin Liu2, Jicheng Zhang3, Ye Qiu4, Lanzhou Li1,2
  • 1Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, College of Plant Protection, Jilin Agricultural University, Changchun 130118, Jilin, China
  • 2School of Life Sciences, Jilin University, Changchun 130012, Jilin, China
  • 3School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA
  • 4Department of Pharmacy, Changchun University of Chinese Medicine, Changchun 130012, Jilin, China
* Equal contribution
Received: September 8, 2023Accepted: November 16, 2023Published: January 3, 2024
https://doi.org/10.18632/aging.205358

Copyright: © 2024 Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Shenzhu Erkang Syrup (SZEK) is a traditional Chinese medicine that improves spleen and stomach function, tonifying the Qi and activating the blood; however, its therapeutic effects in hematopoietic dysfunction and their underlying mechanism remain unexplored. In this study, mice were given cyclophosphamide (100 mg/kg) by intraperitoneal injections for three days to produce hematopoietic dysfunction model. We investigated the hematopoietic effect and mechanism of SZEK in mice with hematopoietic dysfunction via histopathological examination, flow cytometry, enzyme-linked immunosorbent assay, and Western blotting combined with intestinal flora and serum metabolomics analysis. In mice with hematopoietic dysfunction, SZEK (gavage, 0.3 mL/25 g) alleviated pathological damage to the bone marrow and spleen; increased the number of naïve cells (Lin−), hematopoietic stem cells (Lin−Sca-1+c-Kit+), long-term self-renewing hematopoietic stem cells (Lin−Sca-1+c-Kit+CD48−CD150+), B lymphocytes (CD45+CD19+), and macrophages (CD11b+F4/80+) in the bone marrow; and reduced inflammation. Preliminary intestinal flora and serum metabolome analyses indicated that the pro-hematopoietic mechanism of SZEK was associated with macrophage differentiation. Further validation revealed that SZEK promoted hematopoiesis by decreasing the number of M2 macrophages and inhibiting the secretion of negative hematopoietic regulatory factors in mice with hematopoietic dysfunction.