Figure 3.ApoD distribution in lipoproteins. Male
C57BL/6J mice (3-5 weeks old) were fed regular chow (RC) or high fat diet
(HF) for 8 weeks. Aliquots of 250-μl sera of mice (n=5) were fractionated
by gel filtration column chromatography. Fractions (500 μl) were collected
for the determination of cholesterol concentrations. Likewise, aliquots of
sera (250 μl) of male apoE knockout mice (ApoE-/-, 12 weeks old on regular
chow) were fractionated to VLDL, LDL and HDL fractions. Peak fractions of
VLDL, LDL and HDL were subjected to immunoblot assay using anti-ApoD
antibody.
