Research Paper Volume 1, Issue 1 pp 131—145

Extension of chronological life span by reduced TOR signaling requires down-regulation of Sch9p and involves increased mitochondrial OXPHOS complex density

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Figure 1. Elevated mitochondrial translation rates in tor1Δ strains during the exponential and early stationary growth phases. Results of an in vivo-labeling experiment in which the mtDNA-encoded gene products are labeled specifically and visualized by autoradiography after separation by SDS-PAGE (see Materials and Methods). Wild-type (wt) and tor1 null (tor1Δ) strains labeled at mid-log, early stationary (day 1) and later stationary (day 2) are shown. The left-half panel under each time point is the autoradiogram showing the labeled mitochondrial gene products (with each product indicated on the left) and the right-hand panel is the respective Coomassie blue-stained gel as a control for total protein loading.