Research Paper Volume 1, Issue 2 pp 191—211

A screen of apoptosis and senescence regulatory genes for life span effects when over-expressed in Drosophila

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Figure 1. Expression pattern produced by Geneswitch drivers and UAS-GFP reporter in adult flies. The indicated GeneSwitch drivers Act-GS-255B ("255B"), Elav-GS ("Elav") and WB-FB-GS ("FB") were crossed to the UAS-ultraGFP reporter and adult progeny containing both constructs were scored for GFP expression in various tissues. Control flies were generated by crossing UAS-ultraGFP to white1118strain flies to produce progeny containing only UAS-ultraGFP. Age-synchronized flies were cultured in the presence and absence of the drug RU486 for two weeks prior to assay, and GFP expression was scored in whole adult flies and dissected tissues, as indicated. Each image is the overlay of the visible light and GFP images. Insets show details of the regions boxed in white, GFP image only. M = male, F = female. Pictures were taken at the magnification of 20X, 50X, 32X, 20X, 50X, and 80X, for whole fly, head in half, body in half, gut, ovary, and testes, respectively. The white arrow indicates a region of 255B Female flight muscle that is obscured by a fragment of cuticle.