Research Paper Volume 1, Issue 3 pp 303—315

Sod2 haploinsufficiency does not accelerate aging of telomere dysfunctional mice

Figure 3. (A) Number of erythrocytes per ul of peripheral blood ± SEM in 12 to 18 months old mice. (B) Percentage of mature B cells defined as IgD+ IgM+ B220+ CD43- cells in total bone marrow cells of 12 to 18 months old mice. n=21 (G3 mTerc-/-, Sod2+/-), n=17 (G3 mTerc-/-, Sod2+/+); n=9 (mTerc+, Sod2+/-) and n=12 (mTerc+, Sod2+/+) mice per group were analyzed by FACS. (C) Percentage of long term hematopoietic stem cells defined as Lin- Sca+ cKit+ CD34-/low cells in total bone marrow cells of 12 to 18 months old mice. n=9 to 20 mice per group were analyzed by FACS. (D) Competitive transplantation of total bone marrow of Ly5.2 test donor cells against Ly5.1 competitor cells. 8(10)5 cells of test donor cells were transplanted along with 4(10)5 competitor cells into 1 to 3 young lethally irradiated recipients per donor. Four different donors were used per group. White blood cell chimerism was verified at 1, 2 and 5 months after transplantation by FACS analysis. Data is shown as percentage of donor derived chimerism ± SEM (E) Representative pictures displaying the large intestine atrophy in telomere dysfunctional mice wildtype and heterozygous for Sod2. (F) Bar graph depicting the average number of intestinal crypts per visual field at a magnification of 40X of whole mounts from n=8 (G3 mTerc-/-, Sod2+/-), n=7 (G3 mTerc-/-, Sod2+/+); n=4 (mTerc+, Sod2+/-) and n=4 (mTerc+, Sod2+/+) mice per group.